nrf2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc nrf2
Nrf2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+nrf2/NRF2+XP+Rabbit+mAb/pm42863182-43-7-15
Average 98 stars, based on 1572 article reviews

nrf2 - by Bioz Stars, 2026-11

98/100 stars

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Western Blot:

Article Title: Vimentin Cytoskeleton Collapses in Response to the Small Molecule Inhibitor of FH2 domains (SMIFH2)-induced Electrophilic Stress
Article Snippet: The type III intermediate filament protein vimentin plays an integral role in cell homeostasis and disease progression during fibrosis and cancer invasion.. Previous work demonstrated that the pan-formin inhibitor SMIFH2 induced a perinuclear collapse of the vimentin network, suggesting formins may regulate vimentin cytoskeleton organization.. However, despite the designed function of SMIFH2 to inhibit formin FH2 domain-actin interactions, several major off-target effects of SMIFH2 have been reported, including inhibition of myosin family ATPase activity.

Immunofluorescence:

Article Title: α-Synuclein-Related Mitochondria-Nrf2 Dysfunction in Parkinson's Disease Olfactory Mucosa.
Article Snippet: Daniela Maftei,1,2 Maria Grazia Di Certo,3 Riccardo Maurizi,4 Federica Veltri,1 Marco Rosina ,5 Jacopo Bissacco,1 Davide Mascioli,1 Roberta Bovenzi,1 Clara Simonetta,1 Maria Mancini,1 Veronica Buttarazzi,1 Sveva Viola,4 Andrea Gravina,4 Maria Grazia Maglie,4 Anna Maria Rinaldi,1 Francesca Gabanella,3 Luciano Saso,6 Alessandro Stefani,1,2 Francesco Maria Passali,4 Stefano Di Girolamo,4 Mariangela Pierantozzi,1,2 Cristiana Valle,7,8 Alberto Ferri,7,8 Nicola Biagio Mercuri ,1,2 Roberta Lattanzi,6 Cinzia Severini,3‡ and Tommaso Schirinzi, MD, PhD 1,2‡

Incubation:

Article Title: CUL2 confers ferroptosis resistance in pancreatic cancer by disrupting KEAP1-mediated NRF2 degradation.
Article Snippet: Following the respective transfections, cells were fixed with 4% paraformaldehyde for 20 min at room temperature and permeabilized AR TIC LE IN PR ES S with 0.1% Triton X-100 for 15 min. After blocking with 5% BSA for 1h at room temperature, cells were incubated with primary antibodies against E-cadherin (#3195, Cell Signaling Technology, 1:2,000) and N-cadherin (#13116, Cell Signaling Technology, 1:1,000) overnight at 4°C. .. For co-localization analysis, cells were incubated with mouse anti-CUL2 (1:200, Novus, H00008453-M03) and rabbit anti-NRF2 (1:200, Cell Signaling Technology, #12721) primary antibodies overnight at 4°C. .. The next day, cells were washed three times with PBS and incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 594-conjugated goat anti-mouse IgG secondary antibodies (1:500, Invitrogen) for 1h at room temperature in the dark.

Article Title: CUL2 confers ferroptosis resistance in pancreatic cancer by disrupting KEAP1-mediated NRF2 degradation
Article Snippet: Following the respective transfections, cells were fixed with 4% paraformaldehyde for 20 min at room temperature and permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% BSA for 1 h at room temperature, cells were incubated with primary antibodies against E-cadherin (#3195, Cell Signaling Technology, 1:2,000) and N-cadherin (#13116, Cell Signaling Technology, 1:1,000) overnight at 4 °C. .. For co-localization analysis, cells were incubated with mouse anti-CUL2 (1:200, Novus, H00008453-M03) and rabbit anti-NRF2 (1:200, Cell Signaling Technology, #12721) primary antibodies overnight at 4 °C. .. The next day, cells were washed three times with PBS and incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 594-conjugated goat anti-mouse IgG secondary antibodies (1:500, Invitrogen) for 1 h at room temperature in the dark.

Staining:

Blocking Assay:

Binding Assay:

Cell-Signaling:

Article Title: Alpha-ketoglutarate alleviates acute pancreatitis by restoring energy metabolism
Article Snippet: Rabbit anti-ATP5F1A , Boster Biological Technology , Cat# A32267-2. .. Rabbit anti-Nrf2 , Cell Signaling Technology , Cat# 12721T. .. Rabbit anti-KEAP1 , Cell Signaling Technology , Cat# 8047T.

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